Appearance visual inspection. Corn seed embryo shape and color were visually observed. Any kind of embryo protruding or shrunken, dark and dull, the seed is fresh, strong life, can be used for production purposes.
Soaking germination method. 100 seeds were immersed in water for about two hours, swelled, placed on wet grass paper, covered with wet grass paper, placed in ample oxygen, and kept at room temperature of 10-20°C to allow the seeds to fully germinate; Divide by 100 and multiply by 100% to determine the germination rate. Although this method is accurate, it takes 8 days.
Red ink staining method. A commercially available red ink plus 19 copies of tap water was used as a dyeing agent; 100 corn seeds were randomly selected and soaked in water for two hours to allow them to swell; and the emulsified embryos and milk embryos were peeled off with tweezers; After treatment, the seeds were uniformly placed in the incubator, and the dye was injected to submerge the seeds. After dyeing for 15 to 20 minutes, the dye was poured out, and the seeds were washed repeatedly with tap water. Dead embryos and endosperm appear dark red, and live embryos are not stained or slightly light red. Judging the number of live seeds, the number of live seeds is divided by 100 and multiplied by 100% for the germination rate.
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